Methods

Intro to the Lab

Welcome Material  Common Lab Practices  Basics to 18C Fly Stocks

Fly Food

1 liter  2 liter  3 liter  4 liter  5 liter  6 liter  7 liter  8 liter  9 liter  10 liter

Agars

Agar Plates  Egg-lay Caps  Sugar Agar  LB Medium

In situ Protocols

Abdomen Razor Blade Method  P5-P7 Wings  P9-P10 Wings  Probe Making  Wing Development Schedule

Probe Design and Primer Order

Primer Label  Primer Order and Dilution  Probe Design

Isolation, Ligation, injection prep

Isolation of Genomic DNA  Genomic DNA Clean Prep  Genomic DNA Lysis Buffer  Ligation of non-pGEM Vectors  pGEM-TEasy ligation  Midi prep for Fly Egg Injections  Ethanol precipitation of DNA

Competent Cells

Chemical Competent  Electro Competent  Transformation

Longevity and Fecundity Procedures

Longevity and Fecundity  toxicology procedures

Additional User Manuals

NanoDrop 2000  Imaging transgenics  Imager  Making Pipettes

Solutions

SOB Medium  SOC Medium  LB Medium

BCIP NBT  6 x DNA Loading buffer  50 mg/mL Kanamycin  40 mg/mL X-Gal  3 M Na-Acetate pH 5.5  20 x TAE pH 8.0  20 x SSC  20 mM IPTG  220 mg/mL Proteinase K  100 mg/mL Ampicillin  10 x PCR buffer  10 x PBS pH 7.0 - 7.2  10 mg/mL RNAseA  10 mg/mL Proteinase K in PBS

2 M Tris pH 9.5  1 M Tris pH 9.0  1 M Tris pH 8.0  1 M Tris pH 7.9  1 M Tris pH 7.5  1 M Tris pH 7.4

10 mM NaCl   5 M NaCl  5 M Guanidine Hydrochloride  1 M MgCl2  1 M KOH  1 M KCl  75 mM CaCl2  1 M HCl  1 M Glucose  0.5 M EDTA pH 8.0